Download PDF by E.W. Caspari (Ed.): Advances in Genetics, Vol. 19

By E.W. Caspari (Ed.)

ISBN-10: 012017619X

ISBN-13: 9780120176199

This quantity in a chain on genetics, emphasizes the variety of genetic reports. Articles conceal the filamentous fungus neurospora, biogenesis of yeast ribosomes, evolutionary genetics of fish, drosophila transposable parts and the dropophila gene zeste.

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These can be used to distinguish diploid or triploid segregants from haploid ones, since in the former usually a t least one of them remains heterozygous; also loss of homologs containing these mutants can easily be followed. In addition, mutants are generally present only as a single allele which makes identification of all homologs in haploids possible (except for some white haploids, Table 15; the haploids selected on CM+pfp were isolated to check the genotype of the triploid). Only the mutant rib& of group VIII was present in two homologs, since it served as a balancing marker to obtain the heterokaryon between a diploid containing two of the homologs (b/c) and the haploid strain (a) from which the triploid was selected.

In addition, the phenotypes of any viable crossovers are unknown, and their genotypes are usually indistinguishable from those of their second-order nondisjunctional segregants, so that the latter may be mistaken for primary types (Kafer, 1976). Therefore, it usually is impossible to identify the various segregant types before a translocation is well mapped, so that suitable markers can be placed onto the various chromosome segments. 1. Types of Recovered Crossovers and Steps of Segregation a. Recovery and Viability of Primary Crossover Types.

5%) 867 ”Mixed” (proAll) Selected fpaA/fpaA (Fraction) (Nondisjunctionals %) (19 incl. N). 2 . 1 % (6/281) 136 11 % (63/597) 2 . 6 % (13/478) 871 1 . 5%) % Yellow -’% -10% (43 incl. N). 238 ~ . 5%) a suA pro/++ diploids homozygous for yA and adE, were used t o select suA/suA types, among which pro crossovers could not be distinguished from nondisjunctionals (N). 6. yA (and simultaneously adE) distal on I R, and suAadE distal on I L, into pro strains by mitotic crossing-over, followed by haploidisation.

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Advances in Genetics, Vol. 19 by E.W. Caspari (Ed.)


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